Investigating Brunei’s Seafood Markets for Vibrio Parahaemolyticus Using the Most Probable Number- Polymerase Chain Reaction
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منابع مشابه
Prevalence of Listeria monocytogenes, Vibrio parahaemolyticus, Staphylococcus aureus, and Salmonella spp. in seafood products using multiplex polymerase chain reaction.
Although several etiological agents can be transmitted through seafood consumption, Listeria monocytogenes, Vibrio parahaemolyticus, Staphylococcus aureus, and Salmonella spp. are considered among the most important pathogens in terms of public health and disease. In this study, multiplex polymerase chain reaction (PCR), as a rapid and cost-effective method, was used to determine the prevalence...
متن کاملDevelopment of a SYBR green based real-time polymerase chain reaction assay for specific detection and quantification of Vibrio parahaemolyticus from food and environmental samples
Vibrio parahaemolyticus is a foodborne pathogen and their human infection is regularly associated with the consumption of raw or undercooked seafood and contaminated water supplies. Many conventional biochemical identification and confirmation procedures are performed to detect the presence of this pathogen, both from seafood or environmental samples. However, these procedures not only require ...
متن کاملOccurrence and distribution of virulent strains of Vibrio parahaemolyticus in seafoods marketed from Cochin (India)
This study was aimed for the detection of Vibrio parahaemolyticus by biochemical and molecular methods in seafood samples collected from the markets of Cochin located at the southwest coast of India. A total of seventy-two V. parahaemolyticus cultures were isolated by selecting sucrose and cellobiose non-fermenting colonies. All the biochemically confirmed strains were found to have 368-bp toxR...
متن کاملraPid deteCtion of total and PatHogeniC VIBRIo pARAHAemoLyTICus Using real-time PCr witH taqman® flUoresCent Probes
Vibrio parahaemolyticus is found throughout the marine environment and is a major cause of foodborne illness around the world. Foodborne illnesses due to V. parahaemolyticus infections frequently trace back to the consumption of raw or undercooked seafood. The ability to detect V. parahaemolyticus in a rapid, sensi tive, and specific manner is important to safeguard our food supply and prevent...
متن کاملIsolation and characterization of Vibrio parahaemolyticus from seafoods along the southwest coast of India
The work was aimed to study the microbial quality of the seafood sold in the domestic markets and incidence of Vibrio parahaemolyticus. Samples comprising of shellfish, finfish, and cephalopods were collected from various fish markets in and around Cochin. Presumed V. parahaemolyticus were identified by standard biochemical tests, and further confirmed by polymerase chain reaction targeting spe...
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تاریخ انتشار 2013